![]() The positions of SNPs in HEG1 of ACC-MESO-4 are indicated with the reference SNP ID number at the bottom. ![]() HEG1 gene sequences were retrieved from GenBank (accession no. (a) Structure of open reading frame of human HEG1 gene. After detection, the membrane was reprobed and treated with anti-β-actin mAb (AC-15). EGFP-transfected HEK293T (HEK293T + EGFP) was used as a negative control. Cell lysates (5 μ L per lane) were resolved by SDS-PAGE under reducing conditions. (k) Representative western blotting (n = 2) of HEG1-transfected HEK293T (HEK293T + HEG1) using mAb SKM9-2. Control siRNA, MISSION siRNA Universal Negative Control (SIC-001) H1097, H2674, and H3671, HEG1 siRNAs copGFP, a control lentiviral particles. Similar results were obtained by 3 independent analyses. Cell lysates (7.5 μ L per lane) were resolved by SDS-PAGE under reducing conditions and detected by western blotting using mAb SKM9-2. (j) Decrease of SKM9-2 antigen by gene silencing of HEG1. The CBB-stained band was then cut from the gel and analyzed by mass spectrometry and Mascot search. The purified sample was resolved by SDS-PAGE. (i) Results of Mascot search for purified SKM9-2 antigen. ![]() (h) Western blotting and CBB staining of purified SKM9-2 antigen. Pooled fractions are indicated as a black bar. (d,e,f,g) Purification of SKM9-2 antigen using a Superose 6 Increase 10/300GL, Mono Q 5/50GL, WGA-agarose, or Superose 6 Increase 10/300GL column, respectively. A partially purified sample was treated with glycosidase or proteinase K and resolved by SDS-PAGE under reducing conditions. (c) Elimination of recognition with mAb SKM9-2 by glycosidase treatment. An irrelevant mAb (2D2, mouse IgG1) was used as a negative control mAb. (b) Flow cytometric analysis of SKM9-2 antigen on ACCMESO-4. Cell lysates (10 μ L per lane) were resolved by SDS-PAGE under non-reducing conditions. (a) Representative immunoblotting (n = 2) of SKM9-2 antigen in MPM cell lines. Please note that if you purchase something by clicking on a link within this story, we may receive a small commission of the sale.MAb SKM9-2 recognizes a sialylated HEG1 on the cell surfaces of mesothelioma cells. ![]() Our mission at STYLECASTER is to bring style to the people, and we only fe ature products we think you’ll love as much as we do. Just make sure to put your card in before you hit the town! If you really want to go all-in, I won’t blame you for buying multiple to match different handbags. Read on to shop a bunch of card holders that are actually cute. Whether you want a luxe leather look or a basic laminated sleeve that features a funny slogan, this list has it all-and they’re all super affordable, too. Plus, you can find the one that you love and support a small business at the same time- and what’s better than that? I already love the site for its selection of other COVID-friendly pieces like fashion-friendly cloth masks or KN95 options and I am happy to report that the range of vaccine card holders is second-to-none as well. I personally want a vaccine card holder that will stand out in my handbag and will be different from everyone else’s so I don’t get confused on a night out, so heading to Etsy was a no-brainer. Naturally, I turned to Etsy for a bunch of cute options and rounded up the best ones for you to shop right here. I, for one, have been carrying my vaccine card in my wallet for the last few months, so I’m currently on the lookout for a dedicated vaccine card holder so I can upgrade my situation. PSA: You should totally get the COVID-19 vaccine, if you can! And, now that that the vaccines are readily available all across the country, it’s time to focus our attention on making sure that those of us who are vaccinated keep proof on us at all times.
0 Comments
Leave a Reply. |